Antagonistic Activity of Calotropis gigantea and Lactobacillus delbrueckii Against Human Pathogens
S. Rajesh, M. Manohar, Omran M. Abdrhman D. Kannan
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The antagonistic activity of Lactobacillus delbrueckii against target pathogens such as Escherichia coli, Salmonella Sp. Shigella Sp, Aspergillus niger and Candida albicans was tested. The antagonistic activity of Lactobacillus delbrueckii was compared with Calotropis gigantea extract. Antimicrobial activity of Lactobacillus delbrueckii and Calotropis gigantea was assessed by agar well assay technique. Lactobacillus delbrueckii was inoculated into broth and incubated at 370C for 24 hrs. Cell free extracts were obtained by centrifugation. Wells of 6 mm diameter were made in each plate of Tryptone dextrose agar seeded with test pathogenic organisms. Cell free extract of Lactobacillus delbrueckii cultures were transferred at the rate of 50 μl into each well. In another well the plant extract was transferred at the rate of 50 μl. The plates were incubated at 370C for 48 hours without inverting. Zones of inhibition formed around the well were measured.
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